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Addgene

C113S-HA
(Plasmid #113062)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113062 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C113S-KVS-1
  • Species
    C. elegans (nematode)
  • Mutation
    C113S
  • Entrez Gene
    kvs-1 (a.k.a. CELE_C53C9.3)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a K861I mutation in KVS-1 compared to the reference sequence AAO65852.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C113S-HA was a gift from Federico Sesti (Addgene plasmid # 113062 ; http://n2t.net/addgene:113062 ; RRID:Addgene_113062)

  • For your References section:

    Oxidation of potassium channels by ROS: a general mechanism of aging and neurodegeneration?. Sesti F, Liu S, Cai SQ. Trends Cell Biol. 2010 Jan;20(1):45-51. doi: 10.1016/j.tcb.2009.09.008. Epub 2009 Oct 21. 10.1016/j.tcb.2009.09.008 PubMed 19850480
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