KVS1-HA
(Plasmid
#113061)
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PurposeExpresses worm KVS-1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI-neo
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameK+ voltage sensitive channel subunit 1
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SpeciesC. elegans (nematode)
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Entrez Genekvs-1 (a.k.a. CELE_C53C9.3)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains a K861I mutation in KVS-1 compared to the reference sequence AAO65852.1.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KVS1-HA was a gift from Federico Sesti (Addgene plasmid # 113061 ; http://n2t.net/addgene:113061 ; RRID:Addgene_113061) -
For your References section:
MPS-1 is a K+ channel beta-subunit and a serine/threonine kinase. Cai SQ, Hernandez L, Wang Y, Park KH, Sesti F. Nat Neurosci. 2005 Nov;8(11):1503-9. doi: 10.1038/nn1557. Epub 2005 Oct 16. 10.1038/nn1557 PubMed 16222231