MPS-1-cmyc
(Plasmid
#113059)
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PurposeExpresses worm Myc-tagged MPS-1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113059 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI-neo
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMiRP K+ channel accessory Subunit 1
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SpeciesC. elegans (nematode)
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Entrez Genemps-1 (a.k.a. CELE_C29F5.4)
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Tag
/ Fusion Protein
- c-Myc (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid contains an N214K mutation compared to the reference sequence NP_495263.2 that is not of concern for function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MPS-1-cmyc was a gift from Federico Sesti (Addgene plasmid # 113059 ; http://n2t.net/addgene:113059 ; RRID:Addgene_113059) -
For your References section:
Molecular mechanisms underlying KVS-1-MPS-1 complex assembly. Wang Y, Sesti F. Biophys J. 2007 Nov 1;93(9):3083-91. doi: 10.1529/biophysj.107.110320. Epub 2007 Jun 29. 10.1529/biophysj.107.110320 PubMed 17604313