KHT-1
(Plasmid
#113054)
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PurposeExpresses worm KHT-1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113054 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI-neo
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameK+ channel for Habituation to Tap subunit 1
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SpeciesC. elegans (nematode)
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method TOPO Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KHT-1 was a gift from Federico Sesti (Addgene plasmid # 113054 ; http://n2t.net/addgene:113054 ; RRID:Addgene_113054) -
For your References section:
Auto-phosphorylation of a voltage-gated K+ channel controls non-associative learning. Cai SQ, Wang Y, Park KH, Tong X, Pan Z, Sesti F. EMBO J. 2009 Jun 3;28(11):1601-11. doi: 10.1038/emboj.2009.112. Epub 2009 Apr 23. 10.1038/emboj.2009.112 PubMed 19387491