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Addgene

ACPPiso1-V5
(Plasmid #113052)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113052 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    prostatic acid phosphatase isoform 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    ACP3 (a.k.a. 5'-NT, ACP-3, ACPP, TM-PAP)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

  • Cloning method TOPO Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid contains a V360A compared to the reference NP_001090.2 that is not of concern for function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ACPPiso1-V5 was a gift from Federico Sesti (Addgene plasmid # 113052 ; http://n2t.net/addgene:113052 ; RRID:Addgene_113052)

  • For your References section:

    An evolutionarily conserved mode of modulation of Shaw-like K(+) channels. Cotella D, Hernandez-Enriquez B, Duan Z, Wu X, Gazula VR, Brown MR, Kaczmarek LK, Sesti F. FASEB J. 2013 Apr;27(4):1381-93. doi: 10.1096/fj.12-222778. Epub 2012 Dec 11. 10.1096/fj.12-222778 PubMed 23233530
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