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Addgene

Flag-SRm160
(Plasmid #11305)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11305 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SRm160
  • Species
    H. sapiens (human)
  • Entrez Gene
    SRRM1 (a.k.a. 160-KD, POP101, SRM160)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FLAG peptide was inserted between HindIII and BamHI sites. SRm160 (human) was cloned into BamHI and EcoRI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-SRm160 was a gift from Phil Sharp (Addgene plasmid # 11305 ; http://n2t.net/addgene:11305 ; RRID:Addgene_11305)

  • For your References section:

    Regulation of CD44 Alternative Splicing by SRm160 and Its Potential Role in Tumor Cell Invasion. Cheng C, Sharp PA. Mol Cell Biol. 2006 Jan . 26(1):362-70. 10.1128/MCB.26.1.362-370.2006 PubMed 16354706
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