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hSDH5_C83D
(Plasmid #113048)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113048 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET41
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BL-21
  • Growth instructions
    For protein expression (induced with IPTG) grow at 30C but for growing cells without IPTG grow at 37C.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hSDH5_C83D
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    393
  • Mutation
    C83D, Insert begins at amino acid 39
  • GenBank ID
    JF432739.1
  • Entrez Gene
    SDHAF2 (a.k.a. C11orf79, PGL2, SDH5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSDH5_C83D was a gift from Edward A. Berry (Addgene plasmid # 113048 ; http://n2t.net/addgene:113048 ; RRID:Addgene_113048)

  • For your References section:

    In-vitro, SDH5-dependent flavinylation of immobilized human respiratory complex II flavoprotein. Zafreen L, Walker-Kopp N, Huang LS, Berry E. Arch Biochem Biophys. 2016 Aug 15;604:47-56. doi: 10.1016/j.abb.2016.06.006. Epub 2016 Jun 11. 10.1016/j.abb.2016.06.006 PubMed 27296776
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