pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9) Citations (1)

Originally described in: Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9.
Bubeck F, Hoffmann MD, Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Borner K, Fakhiri J, Schmelas C, Dietz L, Grimm D, Correia BE, Eils R, Niopek D Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9. PubMed Journal

Articles Citing pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

Articles
Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins. Hoffmann MD, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri J, Mastel M, Borner K, Eils R, Grimm D, Niopek D. Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271. PubMed Associated Plasmids AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E) AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites) AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold) AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites) More ...

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