CMV-CASANOVA
(Plasmid #113035)

• Purpose: CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
• Depositing Lab: Dominik Niopek
• Publication: Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s41592-018-0178-9. doi: 10.1038/s41592-018-0178-9.

Backbone

• Vector backbone: pcDNA3.1
• Total vector size (bp): 6136
• Vector type: Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
• Alt name: engineered AcrIIA4-LOV2 hybrid for optogenetic control of CRISPR/SpyCas9
• Alt name: inhibits SpyCas9 in the absence of light and releases Cas9 activity upon photoactivation

Resource Information

• Supplemental Documents:
  • 23 Acr_Lov hybrid 4 CASANOVA.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene QC sequence analysis identified a mutation within NeoR/KanR, which results in an early stop codon. The effect of this mutation has not been determined experimentally. Ampicillin should be used for selection in bacteria.