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pST1374-GCN4-dCas9
(Plasmid #113023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113023 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pST1374
  • Backbone size w/o insert (bp) 4968
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCN4-dCas9
  • Alt name
    2xNLS-10xGCN4-NLS-dCas9-NLS
  • Species
    S. cerevisiae (budding yeast); S. pyogenes
  • Insert Size (bp)
    5634
  • Mutation
    D10A, D839A, H840A, N863A
  • Entrez Gene
    GCN4 (a.k.a. YEL009C, AAS101, AAS3, ARG9)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-fwd
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pST1374-GCN4-dCas9 was a gift from Xingxu Huang (Addgene plasmid # 113023 ; http://n2t.net/addgene:113023 ; RRID:Addgene_113023)

  • For your References section:

    BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity. Jiang W, Feng S, Huang S, Yu W, Li G, Yang G, Liu Y, Zhang Y, Zhang L, Hou Y, Chen J, Chen J, Huang X. Cell Res. 2018 Jun 6. pii: 10.1038/s41422-018-0052-4. doi: 10.1038/s41422-018-0052-4. 10.1038/s41422-018-0052-4 [pii] PubMed 29875396
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