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Addgene

IgZ secreted
(Plasmid #11302)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11302 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRII
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3948
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IgZ
  • Alt name
    zeta
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1241
  • GenBank ID
    AY643752
  • Entrez Gene
    ighz (a.k.a. igz, ighm2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Secreted form of zeta. This clone was amplified using primers c2ch1A (5'-CACAGCACCAGTTGTGTTCAA) and c2endD (5'-TAATCTAACGGGTGAGCAACA).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IgZ secreted was a gift from Lisa Steiner (Addgene plasmid # 11302 ; http://n2t.net/addgene:11302 ; RRID:Addgene_11302)

  • For your References section:

    The immunoglobulin heavy-chain locus in zebrafish: identification and expression of a previously unknown isotype, immunoglobulin Z. Danilova N, Bussmann J, Jekosch K, Steiner LA. Nat Immunol. 2005 Mar . 6(3):295-302. 10.1038/ni1166 PubMed 15685175
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