IgM (exon 1-3)
(Plasmid
#11301)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11301 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCRII
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3948
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIgM
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Alt namemu
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)828
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GenBank IDAY643753
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Entrez Geneighm (a.k.a. ighm1, wu:fj14a08, zgc:101769)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Clone A1 (exon 1-3) was amplified using primers zfCH1 (5'-TCAACCATCTGCGCCCCAGT) and cm3A (5'-TCCTGAGGCTTTGCACTT).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
IgM (exon 1-3) was a gift from Lisa Steiner (Addgene plasmid # 11301 ; http://n2t.net/addgene:11301 ; RRID:Addgene_11301) -
For your References section:
The immunoglobulin heavy-chain locus in zebrafish: identification and expression of a previously unknown isotype, immunoglobulin Z. Danilova N, Bussmann J, Jekosch K, Steiner LA. Nat Immunol. 2005 Mar . 6(3):295-302. 10.1038/ni1166 PubMed 15685175
Map uploaded by the depositor.
