pMAL c2x Tral N
(Plasmid
#113009)
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PurposeFor bacterial expression of MBP fusion of N terminal region of Drosophila Tral
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113009 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMAL
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN terminus of tral
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SpeciesD. melanogaster (fly)
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Mutationamino acids 1-355
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Entrez Genetral (a.k.a. Dmel_CG10686, BEST:GH08269, CG10686, DmLSm15, Dmel\CG10686, LSm15, Lsm15, Scd6p, TRAL, Tral, anon-EST:Posey139, anon-WO0172774.89, anon-WO02059370.24, p67, p70)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMAL c2x Tral N was a gift from Terry Orr-Weaver (Addgene plasmid # 113009 ; http://n2t.net/addgene:113009 ; RRID:Addgene_113009) -
For your References section:
Identification of PNG kinase substrates uncovers interactions with the translational repressor TRAL in the oocyte-to-embryo transition. Hara M, Lourido S, Petrova B, Lou HJ, Von Stetina JR, Kashevsky H, Turk BE, Orr-Weaver TL. Elife. 2018 Feb 26;7. pii: 33150. doi: 10.7554/eLife.33150. 10.7554/eLife.33150 PubMed 29480805