pGEX6P-1 gnu 9A
(Plasmid
#113000)
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PurposeFor protein expression and purification of full-length Drosophila gnu 9A
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 113000 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX6P-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefull length gnu
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SpeciesD. melanogaster (fly)
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Mutation9 CycB/CDK1 sites mutated to A
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Entrez Genegnu (a.k.a. Dmel_CG5272, CG5272, Dmel\CG5272, GNU, Gnu)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX6P-1 gnu 9A was a gift from Terry Orr-Weaver (Addgene plasmid # 113000 ; http://n2t.net/addgene:113000 ; RRID:Addgene_113000) -
For your References section:
Control of PNG kinase, a key regulator of mRNA translation, is coupled to meiosis completion at egg activation. Hara M, Petrova B, Orr-Weaver TL. Elife. 2017 May 30;6. doi: 10.7554/eLife.22219. 10.7554/eLife.22219 PubMed 28555567