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Addgene

rag1 short
(Plasmid #11300)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11300 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS(-)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rag1
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    649
  • Entrez Gene
    rag1 (a.k.a. RAG-1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a rag1 PCR product. To make probes: antisense HindIII - T7, sense XbaI - T3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rag1 short was a gift from Lisa Steiner (Addgene plasmid # 11300 ; http://n2t.net/addgene:11300 ; RRID:Addgene_11300)

  • For your References section:

    Characterization and expression of the recombination activating genes (rag1 and rag2) of zebrafish. Willett CE, Cherry JJ, Steiner LA. Immunogenetics. 1997 . 45(6):394-404. 10.1007/s002510050221 PubMed 9089097
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