Yes-10kb
(Plasmid
#112996)
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PurposeP element vector for transposon insertion of the 10Kb Drosophila Amplicon in Follicle Cells (DAFC-62D) into Drosophila genome
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112996 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCasper4
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Vector typeP insertion vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name10Kb DAFC-62D amplicon
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SpeciesD. melanogaster (fly)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Yes-10kb was a gift from Terry Orr-Weaver (Addgene plasmid # 112996 ; http://n2t.net/addgene:112996 ; RRID:Addgene_112996) -
For your References section:
Isolation of a Drosophila amplification origin developmentally activated by transcription. Xie F, Orr-Weaver TL. Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9651-6. doi: 10.1073/pnas.0804146105. Epub 2008 Jul 9. 10.1073/pnas.0804146105 PubMed 18621687