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Addgene

pFastBac His HA GNU WT
(Plasmid #112994)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112994 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin ; Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    To make baculovirus, transform DNA into DH10BAC cells.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gnu
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    gnu (a.k.a. Dmel_CG5272, CG5272, Dmel\CG5272, GNU, Gnu)
  • Tag / Fusion Protein
    • His/HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac His HA GNU WT was a gift from Terry Orr-Weaver (Addgene plasmid # 112994 ; http://n2t.net/addgene:112994 ; RRID:Addgene_112994)

  • For your References section:

    Control of PNG kinase, a key regulator of mRNA translation, is coupled to meiosis completion at egg activation. Hara M, Petrova B, Orr-Weaver TL. Elife. 2017 May 30;6. doi: 10.7554/eLife.22219. 10.7554/eLife.22219 PubMed 28555567
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