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pFastBac Dual PNG172-STII PLU-HIS
(Plasmid #112992)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    DH10BAC pFD
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin ; Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    To make baculovirus, transform DNA into DH10BAC cells.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    png172
  • Species
    D. melanogaster (fly)
  • Mutation
    png172 mutation
  • Entrez Gene
    png (a.k.a. Dmel_CG11420, CG11420, Dmel\CG11420, EG:8D8.5, PNG, Png, fs(1)M2, pan-gnu)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Gene/Insert 2

  • Gene/Insert name
    plu
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    plu (a.k.a. Dmel_CG9183, CG9183, Dmel\CG9183, PLU, Plu)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac Dual PNG172-STII PLU-HIS was a gift from Terry Orr-Weaver (Addgene plasmid # 112992 ; http://n2t.net/addgene:112992 ; RRID:Addgene_112992)
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