6xmyc_cort_UAS-P
(Plasmid
#112991)
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PurposeP element vector for transposon insertion of Myc-tagged Drosophila cort (Cdc20) into Drosophila genome
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112991 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUASp
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Vector typeP insertion vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecort (Cdc20)
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SpeciesD. melanogaster (fly)
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Entrez Genecort (a.k.a. Dmel_CG11330, CG11330, Cort, Dmel\CG11330, cor)
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Tag
/ Fusion Protein
- 6X myc epitope (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site FseI (unknown if destroyed)
- 3′ cloning site AscI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
6xmyc_cort_UAS-P was a gift from Terry Orr-Weaver (Addgene plasmid # 112991 ; http://n2t.net/addgene:112991 ; RRID:Addgene_112991) -
For your References section:
Developmental role and regulation of cortex, a meiosis-specific anaphase-promoting complex/cyclosome activator. Pesin JA, Orr-Weaver TL. PLoS Genet. 2007 Nov;3(11):e202. doi: 10.1371/journal.pgen.0030202. 10.1371/journal.pgen.0030202 PubMed 18020708