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pGex4t2 with 244-458AA SUUR fragment
(Plasmid #112989)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112989 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX 4t2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SuUR
  • Species
    D. melanogaster (fly)
  • Mutation
    amino acids 244-458
  • Entrez Gene
    SuUR (a.k.a. Dmel_CG7869, CG7869, Dmel\CG7869, Dromel_CG7869_FBtr0076203_suur_mORF, SU(UR), SUUR, Su(UR), Su(UR)ES, su(UR)ES)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGex4t2 with 244-458AA SUUR fragment was a gift from Terry Orr-Weaver (Addgene plasmid # 112989 ; http://n2t.net/addgene:112989 ; RRID:Addgene_112989)

  • For your References section:

    DNA copy-number control through inhibition of replication fork progression. Nordman JT, Kozhevnikova EN, Verrijzer CP, Pindyurin AV, Andreyeva EN, Shloma VV, Zhimulev IF, Orr-Weaver TL. Cell Rep. 2014 Nov 6;9(3):841-9. doi: 10.1016/j.celrep.2014.10.005. Epub 2014 Oct 30. 10.1016/j.celrep.2014.10.005 PubMed 25437540
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