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mei-S332-GFP 5.6kb genomic DNA in Casper4 T331A
(Plasmid #112988)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112988 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaSpeR4
  • Vector type
    P insertion vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mei-S332
  • Species
    D. melanogaster (fly)
  • Mutation
    T331A
  • Entrez Gene
    mei-S332 (a.k.a. Dmel_CG5303, BEST:LD13774, CG5303, Dmel\CG5303, MEI-S322, MEI-S332, Mei-S332, Mei-S332/Shugoshin, MeiS332, mei-S322, mei-s332, meiS322, meiS332)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mei-S332-GFP 5.6kb genomic DNA in Casper4 T331A was a gift from Terry Orr-Weaver (Addgene plasmid # 112988 ; http://n2t.net/addgene:112988 ; RRID:Addgene_112988)

  • For your References section:

    Regulation of centromere localization of the Drosophila Shugoshin MEI-S332 and sister-chromatid cohesion in meiosis. Nogueira C, Kashevsky H, Pinto B, Clarke A, Orr-Weaver TL. G3 (Bethesda). 2014 Jul 31;4(10):1849-58. doi: 10.1534/g3.114.012823. 10.1534/g3.114.012823 PubMed 25081981
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