mr in pUASP
(Plasmid
#112981)
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PurposeP element vector for transposon insertion of mr (APC2) into Drosophila genome
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112981 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUASp
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Vector typeP insertion vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemr (APC2)
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SpeciesD. melanogaster (fly)
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Entrez Genemr (a.k.a. Dmel_CG3060, APC2, APC2/mr, Apc2, CG3060, Dmel\CG3060, MR, Mr, dAPC2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mr in pUASP was a gift from Terry Orr-Weaver (Addgene plasmid # 112981 ; http://n2t.net/addgene:112981 ; RRID:Addgene_112981) -
For your References section:
The anaphase promoting complex/cyclosome is required during development for modified cell cycles. Kashevsky H, Wallace JA, Reed BH, Lai C, Hayashi-Hagihara A, Orr-Weaver TL. Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11217-22. doi: 10.1073/pnas.172391099. Epub 2002 Aug 8. 10.1073/pnas.172391099 PubMed 12169670