E2F1 in pGEX4t
(Plasmid
#112976)
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PurposeFor protein expression and purification of Drosophila E2F1
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGEX 4t
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameE2F1
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SpeciesD. melanogaster (fly)
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Entrez GeneE2f1 (a.k.a. Dmel_CG6376, CG6376, DRTF1/E2F, DmE2F-1, Dmel\CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
E2F1 in pGEX4t was a gift from Terry Orr-Weaver (Addgene plasmid # 112976 ; http://n2t.net/addgene:112976 ; RRID:Addgene_112976)