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C-term png in pGEX4
(Plasmid #112975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX 4t
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    png
  • Species
    D. melanogaster (fly)
  • Mutation
    wild-type C terminus
  • Entrez Gene
    png (a.k.a. Dmel_CG11420, CG11420, Dmel\CG11420, EG:8D8.5, PNG, Png, fs(1)M2, pan-gnu)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C-term png in pGEX4 was a gift from Terry Orr-Weaver (Addgene plasmid # 112975 ; http://n2t.net/addgene:112975 ; RRID:Addgene_112975)

  • For your References section:

    PAN GU: a protein kinase that inhibits S phase and promotes mitosis in early Drosophila development. Fenger DD, Carminati JL, Burney-Sigman DL, Kashevsky H, Dines JL, Elfring LK, Orr-Weaver TL. Development. 2000 Nov;127(22):4763-74. PubMed 11044392
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