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Addgene

full length png in pGEX4t
(Plasmid #112974)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112974 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX 4t
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    png
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    900
  • Entrez Gene
    png (a.k.a. Dmel_CG11420, CG11420, Dmel\CG11420, EG:8D8.5, PNG, Png, fs(1)M2, pan-gnu)
  • Tag / Fusion Protein
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    full length png in pGEX4t was a gift from Terry Orr-Weaver (Addgene plasmid # 112974 ; http://n2t.net/addgene:112974 ; RRID:Addgene_112974)

  • For your References section:

    Control of PNG kinase, a key regulator of mRNA translation, is coupled to meiosis completion at egg activation. Hara M, Petrova B, Orr-Weaver TL. Elife. 2017 May 30;6. doi: 10.7554/eLife.22219. 10.7554/eLife.22219 PubMed 28555567
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