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Addgene

GST-GNU-N
(Plasmid #112972)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112972 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gnu
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    gnu (a.k.a. Dmel_CG5272, CG5272, Dmel\CG5272, GNU, Gnu)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Plasmid contains a PPKSDPR deletion at the C-terminus of GST. It is unknown how this deletion affects plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-GNU-N was a gift from Terry Orr-Weaver (Addgene plasmid # 112972 ; http://n2t.net/addgene:112972 ; RRID:Addgene_112972)

  • For your References section:

    The Drosophila cell cycle kinase PAN GU forms an active complex with PLUTONIUM and GNU to regulate embryonic divisions. Lee LA, Van Hoewyk D, Orr-Weaver TL. Genes Dev. 2003 Dec 1;17(23):2979-91. doi: 10.1101/gad.1132603. 10.1101/gad.1132603 PubMed 14665672
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