C2 M2 png-myc in Casper4
(Plasmid
#112968)
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PurposeP element vector for transposon insertion of Myc-tagged png into Drosophila genome
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCaSpeR4
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Vector typeP insertion vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepng-myc
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SpeciesD. melanogaster (fly)
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Entrez Genepng (a.k.a. Dmel_CG11420, CG11420, Dmel\CG11420, EG:8D8.5, PNG, Png, fs(1)M2, pan-gnu)
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Tag
/ Fusion Protein
- myc epitope (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C2 M2 png-myc in Casper4 was a gift from Terry Orr-Weaver (Addgene plasmid # 112968 ; http://n2t.net/addgene:112968 ; RRID:Addgene_112968) -
For your References section:
PAN GU: a protein kinase that inhibits S phase and promotes mitosis in early Drosophila development. Fenger DD, Carminati JL, Burney-Sigman DL, Kashevsky H, Dines JL, Elfring LK, Orr-Weaver TL. Development. 2000 Nov;127(22):4763-74. PubMed 11044392