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UASp-deltaN cycB
(Plasmid #112967)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112967 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASp
  • Vector type
    P insertion vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cycB
  • Alt name
    nondegradable Cyclin B
  • Species
    D. melanogaster (fly)
  • Mutation
    N-term deletion of first 46 amino acids, includes Dbox
  • Entrez Gene
    CycB (a.k.a. Dmel_CG3510, 2g, 3510, CG3510, CYC-B, CYCB, CYCLIN B, Cyc B, Cycb, Cyclin B1, DmcycB, Dmel\CG3510, chr2R:18312808..18312907, cyc B, cyc-B, cycB, cycb, cyclin B, cyclinB, cyclinB1, mAbF2F4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UASp-deltaN cycB was a gift from Terry Orr-Weaver (Addgene plasmid # 112967 ; http://n2t.net/addgene:112967 ; RRID:Addgene_112967)

  • For your References section:

    A genetic screen for suppressors and enhancers of the Drosophila PAN GU cell cycle kinase identifies cyclin B as a target. Lee LA, Elfring LK, Bosco G, Orr-Weaver TL. Genetics. 2001 Aug;158(4):1545-56. PubMed 11514446
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