pCI-Neo-JAM-A
(Plasmid
#112965)
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PurposeTo express feline Jam-A transiently in cell lines that do not naturally express the receptor.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePCI-Neo
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameJunctional Adhesion Molecule type A (JAM-A)
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SpeciesFelis catus
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GenBank ID
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Entrez GeneF11R (a.k.a. JAM-1)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-Neo-JAM-A was a gift from John S. L. Parker (Addgene plasmid # 112965 ; http://n2t.net/addgene:112965 ; RRID:Addgene_112965) -
For your References section:
Conserved Surface Residues on the Feline Calicivirus Capsid Are Essential for Interaction with Its Receptor Feline Junctional Adhesion Molecule A (fJAM-A). Lu Z, Ledgerwood ED, Hinchman MM, Dick R, Parker JSL. J Virol. 2018 Mar 28;92(8):e00035-18. doi: 10.1128/JVI.00035-18. Print 2018 Apr 15. 10.1128/JVI.00035-18 PubMed 29386293
