RhoA sensor (LSS-SGFP2-Scarlet-I)
(Plasmid
#112947)
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PurposeRhoA FRET biosensor
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112947 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTriEX
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRhoA sensor
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SpeciesSynthetic
- Promoter CMV
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Tags
/ Fusion Proteins
- LSS-SGFP2 (N terminal on insert)
- mScarlet-I (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RhoA sensor (LSS-SGFP2-Scarlet-I) was a gift from Dorus Gadella (Addgene plasmid # 112947 ; http://n2t.net/addgene:112947 ; RRID:Addgene_112947)