Lck-mCherry-RGS
(Plasmid
#112935)
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PurposePlasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112935 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep115RhoGEF-RGS domain
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SpeciesSynthetic
- Promoter CMV
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Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lck-mCherry-RGS was a gift from Dorus Gadella (Addgene plasmid # 112935 ; http://n2t.net/addgene:112935 ; RRID:Addgene_112935) -
For your References section:
The balance between Galphai-Cdc42/Rac and Galpha12/13-RhoA pathways determines endothelial barrier regulation by sphingosine-1-phosphate. Reinhard NR, Mastop M, Yin T, Wu Y, Bosma EK, Gadella TWJ Jr, Goedhart J, Hordijk PL. Mol Biol Cell. 2017 Nov 7;28(23):3371-3382. doi: 10.1091/mbc.E17-03-0136. Epub 2017 Sep 27. 10.1091/mbc.E17-03-0136 PubMed 28954861
