G13 sensor (Ga13_2)
(Plasmid
#112930)
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PurposeA FRET sensor for G13 activation, encoded on a single plasmid.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112930 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameG13 sensor
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
G13 sensor (Ga13_2) was a gift from Dorus Gadella (Addgene plasmid # 112930 ; http://n2t.net/addgene:112930 ; RRID:Addgene_112930) -
For your References section:
A FRET-based biosensor for measuring Galpha13 activation in single cells. Mastop M, Reinhard NR, Zuconelli CR, Terwey F, Gadella TWJ Jr, van Unen J, Adjobo-Hermans MJW, Goedhart J. PLoS One. 2018 Mar 5;13(3):e0193705. doi: 10.1371/journal.pone.0193705. eCollection 2018. 10.1371/journal.pone.0193705 PubMed 29505611
