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pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
(Plasmid #112922)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112922 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR
  • Backbone size w/o insert (bp) 2267
  • Total vector size (bp) 11063
  • Vector type
    Mammalian Expression ; Gateway entry vector
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
  • Species
    Synthetic
  • Insert Size (bp)
    8796

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer ATGTAATTCTCCTTGGAATTTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA was a gift from Kosuke Yusa (Addgene plasmid # 112922 ; http://n2t.net/addgene:112922 ; RRID:Addgene_112922)

  • For your References section:

    Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Chong ZS, Ohnishi S, Yusa K, Wright GJ. Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3. 10.1186/s13059-018-1581-3 PubMed 30477585
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