pMLS7-egfp
(Plasmid #112917)

• Purpose: Fluorescence microscopy
• Depositing Lab: Miguel Valvano
• Publication: Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):5956-64.

Backbone

• Vector backbone: pMLS7
• Backbone size w/o insert (bp): 6124
• Total vector size (bp): 6853
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Trimethoprim
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: eGFP
• Insert Size (bp): 735
• Promoter: Ps7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: SmaI (only the plasmid) (destroyed during cloning)
• 5′ sequencing primer: AGCTGGCAACCAATTAGC
• 3′ sequencing primer: pBAD reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry