Skip to main content
Addgene

rd
(Plasmid #112912)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEX-2T
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dematin
  • Alt name
    protein 4.9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1150
  • Mutation
    PEST sequence near the N-terminal removed. 5′-GCGCTCGCTGTCACCCAAAGCCGCAGCGGGGGGCGGAGCCGGGAAGGTGTGGGCGGACAGCCGG-3′, and 5′-CCGGCTGTCCGCCCACACCTTCCCGGCTCCGCCCCCCGCTGCGGCTTTGGGTGACAGCGAGCGC-3′ were used to replace 89KSTSPPPSPEVWAD102 with 89KAAAGGGAGKVWAD102​
  • GenBank ID
    NM_001252664
  • Entrez Gene
    DMTN (a.k.a. DMT, EPB49)
  • Promoter T7
  • Tags / Fusion Proteins
    • Glutatione-S-Tranferase (N terminal on backbone)
    • precision protease site added between GST and dematin

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13_pUC_rev_primer
  • 3′ sequencing primer pGEX_3_primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Athar Chishti, Tufts University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    rd was a gift from C. James McKnight (Addgene plasmid # 112912 ; http://n2t.net/addgene:112912 ; RRID:Addgene_112912)

  • For your References section:

    The allosteric mechanism induced by protein kinase A (PKA) phosphorylation of dematin (band 4.9). Chen L, Brown JW, Mok YF, Hatters DM, McKnight CJ. J Biol Chem. 2013 Mar 22;288(12):8313-20. doi: 10.1074/jbc.M112.438861. Epub 2013 Jan 25. 10.1074/jbc.M112.438861 PubMed 23355471
Related items: