rd-his
(Plasmid #112911)

• Purpose: Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag
• Depositing Lab: C. James McKnight
• Publication: Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20. doi: 10.1074/jbc.M112.438861. Epub 2013 Jan 25.

Backbone

• Vector backbone: pGEX-2T
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: dematin
• Alt name: protein 4.9
• Alt name: 48 kDa form of human dematin
• Species: H. sapiens (human)
• Insert Size (bp): 1150
• Mutation: 89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAGKVWAD102
• GenBank ID: NM_001114137.3
• Entrez Gene: DMTN (a.k.a. DMT, EPB49)
• Promoter: T7
• Tags / Fusion Proteins:
  • Glutathione-S-Transferase (N terminal on backbone)
  • 6-his tag (C terminal on insert)
  • precission protease site between GST and dematin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: M13_pUC_rev_primer
• 3′ sequencing primer: pGEX_3_primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Athar Chishti, Tufts University

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry