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Addgene

pBoL1P4GA
(Plasmid #112910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtU626 PROMOTER-Laz-sgRNA
  • gRNA/shRNA sequence
    To be cloned in
  • Promoter AtU626

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Esp3I (not destroyed)
  • 3′ cloning site Esp3I (not destroyed)
  • 5′ sequencing primer No available
  • 3′ sequencing primer Not available
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBoL1P4GA was a gift from Wendy Harwood (Addgene plasmid # 112910 ; http://n2t.net/addgene:112910 ; RRID:Addgene_112910)

  • For your References section:

    Creating Targeted Gene Knockouts in Brassica oleracea Using CRISPR/Cas9. Lawrenson T, Hundleby P, Harwood W. Methods Mol Biol. 2019;1917:155-170. doi: 10.1007/978-1-4939-8991-1_12. 10.1007/978-1-4939-8991-1_12 PubMed 30610635
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