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Addgene

pBoL1P3GA
(Plasmid #112909)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112909 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AtU626-LACZ-sgRNA
  • gRNA/shRNA sequence
    To be cloned in
  • Species
    A. thaliana (mustard weed)
  • Promoter At U626

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (unknown if destroyed)
  • 3′ cloning site BsaI (unknown if destroyed)
  • 5′ sequencing primer Not available
  • 3′ sequencing primer NOT AVAILABLE
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBoL1P3GA was a gift from Wendy Harwood (Addgene plasmid # 112909 ; http://n2t.net/addgene:112909 ; RRID:Addgene_112909)

  • For your References section:

    Creating Targeted Gene Knockouts in Brassica oleracea Using CRISPR/Cas9. Lawrenson T, Hundleby P, Harwood W. Methods Mol Biol. 2019;1917:155-170. doi: 10.1007/978-1-4939-8991-1_12. 10.1007/978-1-4939-8991-1_12 PubMed 30610635
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