pTORKm43GWF
(Plasmid
#112899)
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Purpose(Empty Backbone) Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mWasabi.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTORKm34GWF
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Backbone manufacturerEdouard Evangelisti, Liron Shenhav
- Backbone size (bp) 8692
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Vector typeOomycete expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/652255v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTORKm43GWF was a gift from Sebastian Schornack (Addgene plasmid # 112899 ; http://n2t.net/addgene:112899 ; RRID:Addgene_112899) -
For your References section:
Hydrodynamic Shape Changes Underpin Nuclear Rerouting in Branched Hyphae of an Oomycete Pathogen. Evangelisti E, Shenhav L, Yunusov T, Le Naour-Vernet M, Rink P, Schornack S. MBio. 2019 Oct 1;10(5). pii: mBio.01516-19. doi: 10.1128/mBio.01516-19. 10.1128/mBio.01516-19 PubMed 31575765
