Skip to main content
Addgene

pGDP4 tet(A)
(Plasmid #112887)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBR322
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    tet(A)
  • Alt name
    tetA
  • Species
    Morganella morganii
  • Mutation
    Sequence was codon optimized for E. coli K12 using the IDT codom optimizing tool
  • Promoter Plac
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ATAAGGGAGAGCGTCGAG
  • 3′ sequencing primer CCAACTCAGCTTCCTTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

JoVE antibiotic dereplication paper: Zubyk, H.L., Cox, G., Wright, G.D. Antibiotic Dereplication Using the Antibiotic Resistance Platform. J. Vis. Exp. (152), e60536, doi:10.3791/60536 (2019). (https://www.jove.com/video/60536/antibiotic-dereplication-using-the-antibiotic-resistance-platform)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGDP4 tet(A) was a gift from Gerard Wright (Addgene plasmid # 112887 ; http://n2t.net/addgene:112887 ; RRID:Addgene_112887)

  • For your References section:

    A Common Platform for Antibiotic Dereplication and Adjuvant Discovery. Cox G, Sieron A, King AM, De Pascale G, Pawlowski AC, Koteva K, Wright GD. Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22. 10.1016/j.chembiol.2016.11.011 PubMed 28017602
Included in kit:
Related items:
Commonly requested with: