pGDP3 rmtB
(Plasmid #112880)

• Purpose: This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
• Depositing Lab: Gerard Wright
• Publication: Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109. doi: 10.1016/j.chembiol.2016.11.011. Epub 2016 Dec 22.

Backbone

• Vector backbone: pBR322
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: rmtB
• Species: Escherichia coli
• Entrez Gene: RmtB (a.k.a. IPF_158)
• Promoter: Pbla
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhel (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CATGAGACAATAACCCTG
• 3′ sequencing primer: CCAACTCAGCTTCCTTTC

Resource Information

• Supplemental Documents:
  • Minimal ARP Protocol
  • Minimal ARP Map

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

JoVE antibiotic dereplication paper: Zubyk, H.L., Cox, G., Wright, G.D. Antibiotic Dereplication Using the Antibiotic Resistance Platform. J. Vis. Exp. (152), e60536, doi:10.3791/60536 (2019). (https://www.jove.com/video/60536/antibiotic-dereplication-using-the-antibiotic-resistance-platform)