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Addgene

pKD4-Nter-APEX2
(Plasmid #112868)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112868 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKD4
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 3267
  • Total vector size (bp) 4053

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    CC118 lambda pir
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    APEX2
  • Species
    Soybean
  • Insert Size (bp)
    771

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CGGAATAGGAACTAAGGAGGATATTCATATGGGAAAGTCTTACCCAACTGTGAGTGC
  • 3′ sequencing primer CGGCTGACATGGGAATTAGCCATGGTCCCCTCCGCCGGCCGCTGCGTCCAGGGTCAGGCGCTCC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKD4-Nter-APEX2 was a gift from Eric Cascales (Addgene plasmid # 112868 ; http://n2t.net/addgene:112868 ; RRID:Addgene_112868)

  • For your References section:

    In vivo TssA proximity labelling during type VI secretion biogenesis reveals TagA as a protein that stops and holds the sheath. Santin YG, Doan T, Lebrun R, Espinosa L, Journet L, Cascales E. Nat Microbiol. 2018 Nov;3(11):1304-1313. doi: 10.1038/s41564-018-0234-3. Epub 2018 Oct 1. 10.1038/s41564-018-0234-3 PubMed 30275513
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