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Addgene

pcDNA5-T/O-Ds1-mCherry
(Plasmid #112855)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/TO
  • Backbone manufacturer
    Thermofisher
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DCHS1
  • Alt name
    dachsous cadherin-related 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    DCHS1 (a.k.a. CDH19, CDH25, CDHR6, FIB1, MVP2, PCDH16, VMLDS1)
  • Promoter doxycycline inducible promoter
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site AgeI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-T/O-Ds1-mCherry was a gift from David Sprinzak (Addgene plasmid # 112855 ; http://n2t.net/addgene:112855 ; RRID:Addgene_112855)

  • For your References section:

    A synthetic planar cell polarity system reveals localized feedback on Fat4-Ds1 complexes. Loza O, Heemskerk I, Gordon-Bar N, Amir-Zilberstein L, Jung Y, Sprinzak D. Elife. 2017 Aug 18;6. pii: 24820. doi: 10.7554/eLife.24820. 24820 [pii] PubMed 28826487
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