pcDNA5-T/O-Ds1-mCherry
(Plasmid #112855)

• Purpose: Dox inducible expression in mammalian cells of Ds1 protein fused to mCherry fluorescent protein
• Depositing Lab: David Sprinzak
• Publication: Loza et al Elife. 2017 Aug 18;6. pii: 24820. doi: 10.7554/eLife.24820.

Backbone

• Vector backbone: pcDNA5/TO
• Backbone manufacturer: Thermofisher
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DCHS1
• Alt name: dachsous cadherin-related 1
• Species: H. sapiens (human)
• Entrez Gene: DCHS1 (a.k.a. CDH19, CDH25, CDHR6, FIB1, MVP2, PCDH16, VMLDS1)
• Promoter: doxycycline inducible promoter
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: AgeI (unknown if destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Supplemental Documents:
  • pCDNA5 TO DS mcherry.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry