pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-4
(Plasmid
#112844)
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Purposetransient/stable overexpression of the N-terminal and central domains of Catulin.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112844 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)/Puro
- Backbone size w/o insert (bp) 5500
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCatulin-alpha-1
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SpeciesM. musculus (mouse)
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MutationN + C1 domains of Catulin
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Entrez GeneCtnnal1 (a.k.a. ACRP, AI616177, AW545119, C86009, Catnal1)
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+)/Puro/FA/GFP-C--Catulin-4 was a gift from Tomasz Prószyński (Addgene plasmid # 112844 ; http://n2t.net/addgene:112844 ; RRID:Addgene_112844) -
For your References section:
Alpha-Dystrobrevin-1 recruits Grb2 and alpha-catulin to organize neurotransmitter receptors at the neuromuscular junction. Gingras J, Gawor M, Bernadzki KM, Grady RM, Hallock P, Glass DJ, Sanes JR, Proszynski TJ. J Cell Sci. 2016 Mar 1;129(5):898-911. doi: 10.1242/jcs.181180. Epub 2016 Jan 14. 10.1242/jcs.181180 PubMed 26769899