FFTTH--GFP-Amotl2
(Plasmid
#112833)
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Purposetransient/stable overexpression
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepShuttle-CMV
- Backbone size w/o insert (bp) 7500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAmotl2
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SpeciesM. musculus (mouse)
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Entrez GeneAmotl2 (a.k.a. Lccp, MASCOT, mKIAA0989)
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
An E371G difference is present in Amotl2 compared to the reference NM_019764.2 that is not of concern for function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FFTTH--GFP-Amotl2 was a gift from Tomasz Prószyński (Addgene plasmid # 112833 ; http://n2t.net/addgene:112833 ; RRID:Addgene_112833)