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Addgene

pcDNA3.1(+)/Puro/FA/FFT-LL5BIP
(Plasmid #112830)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112830 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)/Puro
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LL5BIP
  • Species
    M. musculus (mouse)
  • Entrez Gene
    2410002F23Rik (a.k.a. MGC27669, MGC69849)
  • Tag / Fusion Protein
    • 2xFlag-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)/Puro/FA/FFT-LL5BIP was a gift from Tomasz Prószyński (Addgene plasmid # 112830 ; http://n2t.net/addgene:112830 ; RRID:Addgene_112830)

  • For your References section:

    LL5β interacts with uncharacterised protein 2410002F23Rik (LL5BIP). Niewiadomski P, Prószyński TJ. Matters v. 2, n. 11 (Nov 17, 2016) 10.19185/matters.201610000003
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