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pcDNA3.1(+)/Puro/TagRFP-LL5b
(Plasmid #112828)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112828 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)/Puro
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LL5b
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Phldb2 (a.k.a. AV253284, C820004H04Rik, LL, LL5b, LL5beta)
  • Tag / Fusion Protein
    • TagRFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

LL5b contains a P815S difference compared to NM_153412.4 that does not impact function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)/Puro/TagRFP-LL5b was a gift from Tomasz Prószyński & Joshua Sanes (Addgene plasmid # 112828 ; http://n2t.net/addgene:112828 ; RRID:Addgene_112828)

  • For your References section:

    Amotl2 interacts with LL5beta, localizes to podosomes and regulates postsynaptic differentiation in muscle. Proszynski TJ, Sanes JR. J Cell Sci. 2013 May 15;126(Pt 10):2225-35. doi: 10.1242/jcs.121327. Epub 2013 Mar 22. 10.1242/jcs.121327 PubMed 23525008
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