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Addgene

pRPB1–4stop
(Plasmid #112824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJMD2
  • Backbone size w/o insert (bp) 5345
  • Total vector size (bp) 5957
  • Vector type
    Yeast Expression
  • Selectable markers
    Gentamicin, LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RPB1
  • Alt name
    RPO21
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    5200
  • Mutation
    pSMF2 with repeats 8–10 interrupted with 4 repeats containing stop codons
  • Entrez Gene
    RPO21 (a.k.a. YDL140C, RPB1, RPB220, SIT1, SUA8)
  • Promoter Native promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xma1 (not destroyed)
  • 3′ cloning site Sac1 (not destroyed)
  • 5′ sequencing primer GATCGATGAGGAGTCACTGG
  • 3′ sequencing primer TTTACTAGCGCCGTTGGTTT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRPB1–4stop was a gift from Stephen Fuchs (Addgene plasmid # 112824 ; http://n2t.net/addgene:112824 ; RRID:Addgene_112824)

  • For your References section:

    DNA Instability Maintains the Repeat Length of the Yeast RNA Polymerase II C-terminal Domain. Morrill SA, Exner AE, Babokhov M, Reinfeld BI, Fuchs SM. J Biol Chem. 2016 May 27;291(22):11540-50. doi: 10.1074/jbc.M115.696252. Epub 2016 Mar 29. 10.1074/jbc.M115.696252 PubMed 27026700
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