pGEX-2T-GST-eIF4E K119A
(Plasmid #112818)

• Purpose: Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells
• Depositing Lab: Daniel Schoenberg
• Publication: Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10726-10739. doi: 10.1093/nar/gkx801.

Backbone

• Vector backbone: pGEX-2T
• Backbone manufacturer: GE Healthcare
• Backbone size w/o insert (bp): 4948
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grow in BL21(DE3) cells for protein overexpression
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Eif4e
• Alt name: Eukaryotic translation initiation factor 4E
• Alt name: mRNA cap-binding protein
• Species: M. musculus (mouse)
• Mutation: K119 mutated to A, increasing affinity for cap structure (Spivak-Kroizman et al. 2002)
• Entrez Gene: Eif4e (a.k.a. EG668879, Eif4e-ps, If4e, eIF-4E)
• Promoter: T7
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pGEX-F (CCCATCCTGACTTCATGTTGTATGACGC)
• 3′ sequencing primer: pGEX-R (CGCTACGTGACTGGGTCATGG)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Modified from pGEX-2T-GST-eIF4E provided by Jerry Pelletier, McGill University
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry