p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454)
(Plasmid
#112802)
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PurposeExpression of OCTN2 mutant
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112802 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3 FLAG_CMV14
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Backbone manufacturerSigma
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOCTN2/SLC22A5
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Alt nameOrganic cation/carnitine transporter
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SpeciesR. norvegicus (rat)
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Mutationamino acids 14_22 and 447_454 deleted
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Entrez GeneSlc22a5 (a.k.a. CT1, OCTN2, UST2r)
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Tag
/ Fusion Protein
- 3 x Flag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454) was a gift from Katarzyna Nalęcz (Addgene plasmid # 112802 ; http://n2t.net/addgene:112802 ; RRID:Addgene_112802) -
For your References section:
Caveolin-1--a novel interacting partner of organic cation/carnitine transporter (Octn2): effect of protein kinase C on this interaction in rat astrocytes. Czeredys M, Samluk L, Michalec K, Tulodziecka K, Skowronek K, Nalecz KA. PLoS One. 2013 Dec 13;8(12):e82105. doi: 10.1371/journal.pone.0082105. eCollection 2013. PONE-D-13-35323 [pii] PubMed 24349196